Octacalcium phosphate crystals including a higher density dislocation improve its materials osteogenecity

Herein, we show that the enhanced osteogenecity of octacalcium phosphate (OCP) biomaterial, recently identified as an important element in hybrid organic–inorganic nanocomposites involved initial hydroxyapatite crystal expansion mammal bones, results from chemical property, stemming presence lattice strain and dislocations. Two types OCPs were synthesized by wet-chemical processing (c-OCP) absence (w-OCP) gelatin, respectively, subjected to structural, chemical, biological analyses. High-resolution transmission electron microscopy (HRTEM) fast Fourier transform (FFT) analyses revealed c-OCP crystals contained approximately six times higher edge dislocations with Burgers vectors perpendicular a-axis than case w-OCP. The dissolution tris-HCl buffer occurred toward long axis crystal, most likely, along c-axis direction, while w-OCP dissolved direction. study suggested increment internal energy dislocation density contributed promoting hydrolysis through decreasing activation energy. displayed vitro mesenchymal stem 2D cell 3D spheroid differentiation, vivo bone formation, apatite crystallographic orientation critical-sized rat calvarial defect model compared at same time, converting structure earlier present demonstrates dislocation-related conversion OCP is a determinant induction, which may be relevant normal development utilizing biomaterials.